Multiple headspace solid-phase microextraction (MHS-SPME) methodology applied to the determination of volatile metabolites of plasticizers in human urine

• Validation of MHS-SPME-GC-MS method for the analysis two plasticizer metabolites. No matrix effect, no external calibration, loss, solvents free and quick method. Application to urine samples population with exposure plastics. In present work, an optimization validation analytical determination metabolites, 2-ethylhexanol 4-heptanone in urine, were carried out by multiple headspace solid-phase microextraction (MHS-SPME) coupled GC–MS. The study was successfully performed terms stability, selectivity, linearity, accuracy, recovery, intermediate precision, repeatability, limit detection (LOD) quantification (LOQ) samples. Finally, group studies developed volunteers plastic exposure. First represents common general second one healthy moderately trained individuals who have received blood transfusion. This demonstrates that significantly increased levels found (p < 0.05) early hours after receiving